Fine structure mapping of an avian tumor virus RNA by immunoelectron microscopy
نویسندگان
چکیده
منابع مشابه
Fine structure mapping of an avian tumor virus RNA by immunoelectron microscopy.
The RNA of a deleted strain (lacking Src gene) of an avian sarcoma virus (ASV) was examined by a newly developed immunoelectron microscopic procedure which uses anti-nucleotide antibodies as probes. After denaturation of the RNA and reaction with a high affinity, highly specific anti-7-methylguanosine-5'-phosphate (anti-pm 7G), 81% of 106 molecules examined were found to have antibody at one te...
متن کاملStudies on mouse mammary tumor virus (MTV) and mouse leukemia virus (MuLV) by immunoelectron microscopy.
Antigenic properties of the mouse mammary tumor virus (MTV or type B) particles and of the murine leukemia virus (MuLV or type C) particles have been studied by immunoelectron microscopy. Mouse mammary tumor cell lines producing either both type B and type C virus particles or only B or C type particles have been used. Mouse reticulum cell sarcoma and rat bone tumor cell lines producing type C ...
متن کاملQuantitation of avian RNA tumor virus reverse transcriptase by radioimmunoassay.
A radioimmunoassay was developed that can detect and quantitate 3 ng or more of the avian RNA tumor virus reverse transcriptase. The assay detected no antigenic sites in Rous sarcoma virus alpha virions or in virions of a murine RNA tumor virus. About 70 molecules of reverse transcriptase were found per virion of avian myleloblastosis virus with this assay or with an assay based on antibody inh...
متن کاملAvian reticuloendotheliosis virus: characterization of genome structure by heteroduplex mapping.
The genome structure of defective, oncogenic avian reticuloendotheliosis virus (REV) was studied by heteroduplex mapping between the full-length complementary DNA of the helper virus REV-T1 and the 30S REV RNA. The REV genome (5.5 kilobases) had a deletion of 3.69 kilobases in the gag-pol region, confirming the genetic defectiveness of REV. In addition, REV lacked the sequences corresponding to...
متن کاملComparison of an avian osteopetrosis virus with an avian lymphomatosis virus by RNA-DNA hybridization.
Myeloblastosis-associated virus (MAV)-2(0), a virus which was derived from avian myeloblastosis virus and induced a high incidence of osteopetrosis, was compared with avian lymphomatosis virus 5938, a recent field isolate which induced a high incidence of lymphomatosis. The following information was obtained. (i) MAV-2(0) induced osteopetrosis, nephroblastoma, and a very low incidence of hepato...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1980
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/8.19.4485